Description
The SARS-CoV-2 Antigen Quantitative ELISA uses the principle of the double antibody sandwich method to detect SARS-CoV-2 N protein in human serum or plasma. Anti-SARS-CoV-2 N protein antibody was used to prepare microplates in advance. Add the biotin-labelled anti-SARS-CoV-2 N protein antibody and the sample in sequence. If the sample contains N protein, a solid-phase antibody-antigen-biotin-labelled antibody complex is formed. After washing the microplate, streptavidin labelled with HRP is added to further form an immune complex. The unbound substances are washed away, and a substrate solution containing TMB and urea hydrogen peroxide is added to the microplate. The wells are blue in color, and after being stopped by the stop solution, they can turn into yellow. The absorbance value was read using a microplate ELISA reader with a wavelength of 450 nm, using 620nm to 690nm as the reference wavelength. And the content of SARS-CoV-2 N protein in the sample was calculated according to the concentration of the SARS-CoV-2 calibrator